Anti-SGLT1 antibody ab14686 from Abcam

Product Specs
ItemAnti-SGLT1 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Supplier Product Page
Catalog Numberab14686
Antibody TypePrimary
Quantity100 UG
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityPolyclonal
IsotypeIgG
HostRabbit
Conjugate/TagUnconjugated
Concentration0.6 mg/ml
Gene NameSLC5A1
ReactivityHuman
TargetSGLT1
FormatPurified on antigen sepharose affinity column

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Abcam

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Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Regulatory mechanisms of glucose absorption in the mouse proximal small intestine during fasting and feedingScientific reportsJuly 5, 2023Nakamura C, Ishizuka N, Yokoyama K, Yazaki Y, Tatsumi F, Ikumi N, Hempstock W, Ikari A, Yoshino Y, Hayashi H.are directed against a synthetic peptide corresponding to human SGLT1, from amino acids 600 to 700; ab14686, 1:200 dilution: Abcam, Antibodies are directed against a synthetic peptide corresponding to human SGLT1,

(2) Alleviative Effect of Probiotic Ferment on Lawsonia intracellularis Infection in PigletsBiology (Basel)June 17, 2023Tingting Xu, Yong Guo, Yuanyuan Zhang, Kai Cao, Xinchen Zhou, Mengqi Qian, Xinyan Hanantibody (diluted at 1:800, Proteintech 14195-1-AP), anti-SGLT1 antibody (diluted at 1: 500, Abcam ab14686), anti-TCN2 antibody (diluted at 1: 200, LSBio LS-C373504), and anti-β-actin antibody (Abcam ab68477).

(3) Oral metformin transiently lowers post-prandial glucose response by reducing the apical expression of sodium-glucose co-transporter 1 in enterocytesiScienceApril 21, 2023Lorea Zubiaga, Olivier Briand, Florent Auger, Veronique Touche, Thomas Hubert, Julien Thevenet, Camille Marciniak, Audrey Quenon, Caroline Bonner, Simon Peschard, et al.(1:100),Millipore,"Cat# 07-1417, RRID: AB_1587521" Rabbit Polyclonal anti-SGLT1 (1:250),Abcam,Cat#Ab14686 Mouse Monoclonal anti-ZO1 (1:50),Invitrogen,"Cat# 33-9100,RRID: AB_2533147" Rabbit Polyclonal anti-AMPKα

(4) Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Paeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysisPharm BiolDecember 1, 2022Zhang Y, Niu J, Zhang S, Si X, Bian TT, Wu H, Li D, Sun Y, Jia J, Xin E, et al.(1:500 dilutions, 20436-1-AP, Proteintech Group, Inc., Rosemont, IL, USA) and SGLT1 (1:800 dilutions, ab14686, Abcam, Cambridge, UK) overnight at 4°C. After washing with TBST, the membrane was incubated with the

(5) Lupinus albus γ-Conglutin: New Findings about Its Action at the Intestinal Barrier and a Critical Analysis of the State of the Art on Its Postprandial Glycaemic Regulating ActivityNutrientsSeptember 5, 2022Giuditta C. Heinzl, Marco Tretola, Stefano De Benedetti, Paolo Silacci, Alessio Scarafonialbumin. The following primary antibodies were used to incubate the membranes at 4 °C overnight: SGLT1 (Ab14686, Abcam plc, Cambridge, UK) diluted 1:500 in 0.10% Tween, in 1X PBS containing 5% bovine serum albumin

(6) Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID)Journal of clinical medicineJuly 19, 2022Ahsan MK, Dos Reis DC, Barbieri A, Sumigray KD, Nottoli T, Salas PJ, Ameen NA.(Burlington, MA, USA), Phospho-PKCιThr563 (iota; #GTX25813) from GeneTex (San Antonio, TX, USA), Anti-SGLT1 (#ab14686) from Abcam (Cambridge, UK), and Myosin VB (#NBP1-87746) from Novus Biologicals (Centennial, CO, USA).

(7) Enterocyte HKDC1 Modulates Intestinal Glucose Absorption in Male Mice Fed a High-fat DietEndocrinologyJune 1, 2022Zapater JL, Wicksteed B, Layden BT.nitrocellulose membranes as detailed above. Membranes were blocked as above, then incubated with SGLT1 (#ab14686, RRID https://scicrunch.org/resolver/RRID:AB_301411, Abcam, Princeton, NJ, USA), GLUT2 (#sc-518022,

(8) Empagliflozin inhibits angiotensin II-induced hypertrophy in H9c2 cardiomyoblasts through inhibition of NHE1 expressionMolecular and Cellular BiochemistryJune 1, 2022Nabeel Abdulrahman et al.USA), Fisher Scientific (Waltham, MA, USA), or Sigma (St. Louis, MO, USA). Primary antibodies SGLT-1 (ab14686) polyclonal, SGLT-2 (ab85626) polyclonal, NHE1 (ab126725) monoclonal, alpha tubulin (catalog no. ab4074)

(9) The sodium/glucose cotransporters as potential therapeutic targets for CF lung diseases revealed by human lung organoid swelling assayMolecular therapy. Methods & clinical developmentMarch 10, 2022Hirai H, Liang X, Sun Y, Zhang Y, Zhang J, Chen YE, Mou H, Zhao Y, Xu J.prepared from ALI culture at 16 days for western blot analysis to quantify SGLT1 expression (1:400-1:500, ab14686, Abcam, Cambridge, UK). Immunofluorescence staining Cells were fixed with 4% paraformaldehyde (PFA)

(10) HIF-1α Alleviates High-Glucose-Induced Renal Tubular Cell Injury by Promoting Parkin/PINK1-Mediated MitophagyFrontiers in MedicineFebruary 3, 2022Lu Yu, Yulin Wang, Yan Hong Guo, Liuwei Wang, Zijun Yang, Zi Han Zhai, Lin TangAnti-Fibronectin/FN (1:2000, CST, #26836), Anti-a-SMA (1:2000, CST, # 19245), and Anti-SGLT1 (1:2000, Abcam, ab14686). The experiments were repeated more than three times, and the images were captured after incubation

(11) The preference for sugar over sweetener depends on a gut sensor cellNature neuroscienceFebruary 1, 2022Buchanan KL, Rupprecht LE, Kaelberer MM, Sahasrabudhe A, Klein ME, Villalobos JA, Liu WW, Yang A, Gelman J, Park S, et al.room temperature. The following primary antibodies and dilutions were used: anti-SGLT1 (rabbit, Abcam, ab14686; 1:100) and anti-GFP (chicken, Abcam, ab13970; 1:500). After primary antibody incubation, tissue was

(12) Berberine Decreases Intestinal GLUT2 Translocation and Reduces Intestinal Glucose Absorption in MiceInternational Journal of Molecular SciencesDecember 28, 2021Min Zhang, Hongyan Yang, Erwan Yang, Jia Li, Ling DongSC-9117, 1:500, Dallas, TX, USA), GLUT5 (Santa Cruz, SC-30109, 1:500, Dallas, TX, USA), SGLT1 (Abcam, ab14686, 1:500, Waltham, MA, USA), PLC-β2 (Santa Cruz, SC-206, 1:500, Dallas, TX, USA), IGF-1R-β (Cell Signaling Technologies

(13) Changes in porcine nutrient transport physiology in response to Ascaris suum infectionParasites & vectorsOctober 14, 2021Koehler S, Springer A, Issel N, Klinger S, Strube C, Breves G.used in western blot experiments AM apical membrane, CM crude membrane, CY cytosolaRabbit-anti-SGLT1 (ab14686, Abcam, Cambridge, UK)bCustom-made (Perbio Science, Bonn, Germany; epitope: KIRKRApSEKELMI)cRabbit-anti-ASCT1

(14) SGLT1 Knockdown Attenuates Cardiac Fibroblast Activation in Diabetic Cardiac FibrosisFrontiers in PharmacologyJune 24, 2021Hui Lin, Le Guan, Liping Meng, Hiroyasu Uzui, Hangyuan Guoprimary antibodies were purchased from Abcam and used at a 1:1,000 dilution: β-actin (ab5694), SGLT1 (ab14686), collagen I (ab34710), and collagen III (ab6310). The following antibodies were purchased from Cell Signaling Technology

(15) A Simple Method for Creating a High‐Content Microscope for Imaging Multiplexed Tissue MicroarraysCurrent ProtocolsApril 6, 2021Shabnam Abtahi, Neal R. Gliksman, John F. Heneghan, Steven P. Nilsen, Jeremy L. Muhlich, Jay Copeland, Emil Rozbicki, Chris Allan, Pradeep K. Dudeja, Jerrold R. Turnerμg/ml,AB_2783812 CLDN15,Rabbit,Polyclonal,Thermo Fisher,38-9200,QJ228299,1.7 μg/ml,AB_2533391 SLC5A1,Rabbit,Polyclonal,Abcam,Ab14686,GR140673-6,6 μg/ml,AB_301411 ZO-1,Rabbit,Polyclonal,Thermo Fisher,61-7300,1452279A,0.5 μg/ml,AB_138452 SLC9A3,Rabbit,Polyclonal,Abcam,Ab101817,GR53426-4,1:100,AB_10715387 Na+-K+

(16) Caco-2/HT29-MTX co-cultured cells as a model for studying physiological properties and toxin-induced effects on intestinal cellsPloS oneJanuary 1, 2021Hoffmann P, Burmester M, Langeheine M, Brehm R, Empl MT, Seeger B, Breves G.min.1 monoclonal mouse-anti-PEPT1 (373742, Santa Cruz, lot no.: 31916).2 polyclonal rabbit- anti-SGLT1 (ab14686, Abcam, UK, lot no.: JBC17887303).3 monoclonal rabbit-antipSGLT1, custom made by Perbio Science, Germany

(17) Sodium/glucose Co-Transporter 2 Inhibitor, Empagliflozin, Alleviated Transient Expression of SGLT2 after Myocardial InfarctionKorean Circulation JournalJanuary 1, 2021Soo Yong Lee et al.room temperature. Primary antibodies used in this study were as follows: anti-SGLT1 antibody (Abcam, ab14686), anti-SGLT2 (Abcam, ab37296), anti-Hypoxia-inducible Factor-1α (HIF-1α) (SantaCruz, sc-10790), anti-transforming

(18) FGF21 Mimics a Fasting-Induced Metabolic State and Increases Appetite in ZebrafishScientific ReportsDecember 1, 2020Ayelén Melisa Blanco, Juan Ignacio Bertucci, Suraj Unniappan(1:500 dilution; Catalog # ab111117; Abcam) and rabbit polyclonal to SGLT1 (1:500 dilution; Catalog # ab14686; Abcam). Vinculin protein was used for normalization and was detected using rabbit antiserum directed

(19) Feeding and food availability modulate brain-derived neurotrophic factor, an orexigen with metabolic roles in zebrafishScientific ReportsDecember 1, 2020Ayelén Melisa Blanco, Juan Ignacio Bertucci, Azadeh Hatef, Suraj Unniappan(1:500 dilution; Catalog # ab111117; Abcam) and rabbit polyclonal to SGLT1 (1:500 dilution; Catalog # ab14686; Abcam). Vinculin protein was used for normalization and was detected using rabbit antiserum directed

(20) Molecular Mechanisms Underlying the Absorption of Aglycone and Glycosidic Flavonoids in a Caco-2 BBe1 Cell ModelACS OmegaMay 19, 2020Hua Zhang, Yousef I. Hassan, Ronghua Liu, Lili Mats, Cheng Yang, Chunming Liu, Rong Tsaounless otherwise specified. Western blotting was carried out using the following antibodies: anti-SGLT1 (ab14686) (Abcam Inc., Toronto, ON, Canada) and CST-β-actin (13 × 105) (Cell Signaling Technology, Inc., Danvers,

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(1)

2/27/2020Detection of SGLT1 in Mouse Brain Lisha JoshiA good Ab for the detection of SGLT1 expression in mouse brain.

Anti-SGLT1 antibody from Abcam

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Anti-SGLT1 antibody from Abcam

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